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UltraMAB

NBN UltraMAB Antibody, Clone UMAB99

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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SKU Description Datasheet Price Availability*  
UM800029
  • NBN mouse monoclonal antibody,clone UMAB99, 100 ul
$468 In Stock Add to Shopping Cart
UM870029
  • NBN mouse monoclonal antibody,clone UMAB99, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
Data Img
IHC(7)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
Gene NameHomo sapiens nibrin (NBN)
SynonymsAT-V1; AT-V2; ATV; NBS; NBS1; P95
ImmunogenHuman recombinant protein fragment corresponding to amino acids 461-754 of human NBN (NP_002476) produced in E.coli.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB99 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsIHC 1:100, IF 1:100
BackgroundMutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008].

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NBN monoclonal antibody (Clone UMAB99).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-$NBNmouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of A549 cells using NBN mouse monoclonal antibody (UM800029, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NBN mouse monoclonal antibody (UUM800029). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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