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UltraMAB

NME1 UltraMAB Antibody, Clone UMAB94

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM800025
  • NME1 mouse monoclonal antibody,clone UMAB94, 100 ul
468 In Stock Add to Shopping Cart
UM870025
  • NME1 mouse monoclonal antibody,clone UMAB94, 30 ul
$120 In Stock Add to Shopping Cart
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
WB(1)
IHC(8)
IF(1)
10K-CHIP(1)
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Also for NME1 (NM_198175)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens NME/NM23 nucleoside diphosphate kinase 1 (NME1), transcript variant 1
SynonymsAWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1
ImmunogenFull length human recombinant protein of human NME1 (NP_937818) produced in E.coli.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB94 IsotypeIgG1
Species ReactivityHuman, Rat, Monkey, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsIHC 1:100, IF 1:100
BackgroundThis gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008].

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WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NME1 monoclonal antibody (Clone UMAB94).
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IF Image
Immunofluorescent staining of A549 cells using NME1 mouse monoclonal antibody (UM800025, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NME1 mouse monoclonal antibody (UM800025). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

 

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