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BID UltraMAB Antibody, Clone UMAB139
UltraMAB Antibodies - Validated with 10K Protein Chip
|Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|| WB 1:2000, IHC 1:200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2|
|This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]. |
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Western blot analysis of HEK293 cell lysate (35ug) by using anti-BID monoclonal antibody.
Western blot analysis of COLO205 cell lysate (35ug) by using anti-BID monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BID monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot analysis of DU145 cell lysate (35ug) by using anti-BID monoclonal antibody UM500101
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)