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MRPS34 UltraMAB Antibody, Clone UMAB125
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for MRPS34 (NM_023936)
|Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|| IHC 1:100~200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens mitochondrial ribosomal protein S34 (MRPS34), transcript variant 2|
|MRP-S12; MRP-S34; MRPS12|
|Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]. |
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS34 mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS34mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS34 mouse monoclonal antibody. (UM500090; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of HeLa cells using anti-MRPS34 mouse monoclonal antibody (UM500090, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MRPS34 mouse monoclonal antibody (UM500090). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.