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UltraMAB

BBOX1 UltraMAB Antibody, Clone Clone UMAB119

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500084
  • BBOX1 mouse monoclonal antibody,clone UMAB119, 100 ul
$468 In Stock Add to Shopping Cart
UM570084
  • BBOX1 mouse monoclonal antibody,clone UMAB119, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
Data Img
IHC(3)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
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Also for BBOX1 (NM_003986)
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OriGene Data

ImmunogenFull length human recombinant protein of human BBOX1 (NP_003977) produced in HEK293T cell.
Clone NameClone Clone UMAB119 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested Dilutions IHC 1:100~200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1 (BBOX1)
Alternative NameBBH; BBOX; G-BBH; gamma-BBH
Database LinkNP_003977
FunctionThis gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BBOX1 mouse monoclonal antibody. (UM500084; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human breast duct using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic, membrane, and nuclear staining in ductal epithelial cells.
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Immunohistochemical staining of paraffin-embedded human kidney carcinoma using anti-BBOX1 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500084 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The composit image shows strong cytoplasmic, membrane, and nuclear in panel A tumor cells and no staining in panel B tumor cells but strong staining is normal tubule epithelial cells..
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Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (UM500084, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BBOX1 mouse monoclonal antibody (UM500084). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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