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UltraMAB

IRF6 UltraMAB Antibody, Clone UMAB106

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500074
  • IRF6 mouse monoclonal antibody,clone UMAB106, 100 ul
$468 In Stock Add to Shopping Cart
UM570074
  • IRF6 mouse monoclonal antibody,clone UMAB106, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
Data Img
IHC(6)
Data Img
IF(1)
Data Img
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OriGene Data

ImmunogenFull length human recombinant protein of human IRF6(NP_006138) produced in HEK293 Cell.
Clone NameClone UMAB106 IsotypeIgG2b
Species ReactivityHuman, Monkey, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500, IHC 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens interferon regulatory factor 6 (IRF6), transcript variant 1
Alternative NameLPS; OFC6; PIT; PPS; PPS1; VWS; VWS1
Database LinkNP_006138
FunctionThis gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Immunohistochemical staining of paraffin-embedded human tonsil composit using anti-IRF6 clone UMAB106 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500074); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in both the 20 and 40x however the 20x reveal the the squamous epithelial are positive for IRF6 and the cells of the germinal and non germinal centers are negative for IRF6.
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Immunohistochemical staining of paraffin-embedded human endometrium using anti-IRF6 clone UMAB106 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500074); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in placenta.
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Immunohistochemical staining of paraffin-embedded human placenta using anti-IRF6 clone UMAB106 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500074); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in placenta.
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IRF6 mouse monoclonal antibody. (UM500074; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody. (UM500074; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IRF6 mouse monoclonal antibody. (UM500074; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of HeLa cells using anti-IRF6 mouse monoclonal antibody (UM500074, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
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