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UltraMAB

EGFR UltraMAB Antibody, Clone UMAB95

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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SKU Description Datasheet Price Availability*  
UM500070
  • EGFR mouse monoclonal antibody,clone UMAB95, 100 ul
$468 In Stock Add to Shopping Cart
UM570070
  • EGFR mouse monoclonal antibody,clone UMAB95, 30 ul
$120 In Stock Add to Shopping Cart

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details

WB(1)
Data Img
IHC(2)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
Gene NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 3
SynonymsERBB; ERBB1; HER1; mENA; NISBD2; PIG61
ImmunogenFull length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB95 IsotypeIgG1
Species ReactivityHuman, Monkey, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:500, IHC 1:100
BackgroundThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010].

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EGFR monoclonal antibody (Clone UMAB95).
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of HeLa cells using anti-EGFR mouse monoclonal antibody (UM500070, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EGFR mouse monoclonal antibody (UM500070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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