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ALDH1L1 UltraMAB Antibody, Clone UMAB45
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for ALDH1L1 (NM_012190)
|Full length human recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), transcript variant 2|
|10-fTHF; 10-FTHFDH; FDH; FTHFD|
|The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]. |
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody (Clone UMAB45).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Western blot of mouse tissue lysates (20ug) from Liver. Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-ALDH1L1 mouse monoclonal antibody. (UM500041; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-ALDH1L1 mouse monoclonal antibody. (UM500041; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-ALDH1L1 mouse monoclonal antibody. (UM500041; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of HepG2 cells using ALDH1L1 mouse monoclonal antibody (UM500041, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Immunofluorescent staining of SH-SY5Y cells using anti-ALDH1L1 mouse monoclonal antibody (UM500041, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.