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UltraMAB

HER2 UltraMAB Antibody, Clone UMAB35

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500035
  • Purified HER2 (ERBB2) mouse monoclonal antibody,clone UMAB35, 100 ul
468 In Stock Add to Shopping Cart
UM570035
  • ERBB2 mouse monoclonal antibody,clone UMAB35, 30 ul
$120 In Stock Add to Shopping Cart
WB(3)
IHC(2)
IF(2)
10K-CHIP(1)
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Also for HER2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
SynonymsCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
ImmunogenHuman recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB35 IsotypeIgG2b
Species ReactivityHuman, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB:1:500, IHC:1:50, IF:1:100
BackgroundThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008].

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500035, clone UMAB35, 1:500).
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB35) at 1:500.
WB Image
Western Blot analysis of 10 different human tissue lysates (35ug) by using anti-HER2 monoclonal antibody (clone UMAB35, 1:500)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB35, Dilution 1:100)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++) using anti-ERBB2mouse monoclonal antibody. (Clone UMAB35, Dilution 1:100)
IF Image
Anti-ERBB2 mouse monoclonal antibody (UM500035, clone UMAB35, 1:100) immunofluorescent staining of COLO205 cells.
IF Image
Anti-ERBB2 mouse monoclonal antibody (UM500035, clone UMAB35, 1:100) immunofluorescent staining of MCF7 cells.
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB35). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB35) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.

 

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