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UltraMAB

FOLH1 UltraMAB Antibody, Clone UMAB25

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500025
  • FOLH1 mouse monoclonal antibody,clone UMAB25, 100 ul
$468 In Stock Add to Shopping Cart
UM570025
  • FOLH1 mouse monoclonal antibody,clone UMAB25, 30 ul
$120 In Stock Add to Shopping Cart

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details

WB(1)
Data Img
IHC(8)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
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Also for FOLH1 (NM_004476)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
Gene NameHomo sapiens folate hydrolase (prostate-specific membrane antigen) 1 (FOLH1), transcript variant 1
SynonymsFGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA
ImmunogenFull length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB25 IsotypeIgG2b
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:500~2000, IF 1:100, IF 1:100
BackgroundThis gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq].

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* OriGene provides validated application data and protocol, with money back guarantee.

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-FOLH1 monoclonal antibody (clone UMAB25, 1:500)
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Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded prostate tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of PC-12 cells using FOLH1 mouse monoclonal antibody (UM500025, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOLH1 mouse monoclonal antibody (Clone UMAB25). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-FOLH1 (Clone UMAB25) very specifically recognizes FOLH1 antigen on OriGene protein microarray chip.
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