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UltraMAB

CDH2 UltraMAB Antibody, Clone UMAB23

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500023
  • CDH2 mouse monoclonal antibody,clone UMAB23, 100 ul
$468 In Stock Add to Shopping Cart
UM570023
  • CDH2 mouse monoclonal antibody,clone UMAB23, 30 ul
$120 In Stock Add to Shopping Cart
WB(3)
Data Img
IHC(8)
Data Img
IF(1)
Data Img
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OriGene Data

ImmunogenFull length human recombinant protein of human CDH2(NP_001783) produced in HEK293T cell.
Clone NameClone UMAB23 IsotypeIgG2a
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens cadherin 2, type 1, N-cadherin (neuronal) (CDH2)
Alternative NameCD325; CDHN; CDw325; NCAD
Database LinkNP_001783
FunctionThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of mouse tissue lysates (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
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Western Blot analysis of extracts (35µg) from 11 different cell lines by using anti-CDH2 monoclonal antibody (Clone UMAB23)
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Immunohistochemical staining of paraffin-embedded of human thyroid carcinoma using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells
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Immunohistochemical staining of paraffin-embedded of human kidney using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining in the kidney tubules
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Immunohistochemical staining of paraffin-embedded of human ovarian carcinoma using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the tumor cells
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Immunohistochemical staining of paraffin-embedded of human endometrium using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong membranous and cytoplsamic staining of the endometrial cells.
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Immunohistochemical staining of paraffin-embedded of human carcinoma of the lung using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows the tumor cells with membraneous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded of human lymph node using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows cells of the lymph node are negative. Positive staining seen in the endothelial cells, mostly membraneous with weak cytoplasmic .
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Immunohistochemical staining of paraffin-embedded of human pancreas using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows gladular cells of the pancreas membraneous and cytoplasmic staining cells.
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Immunohistochemical staining of paraffin-embedded of 3 human carcinoma of kidney using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human carcinoma of the kidney all show strong membraneous and cytoplasmic staining in the tumor cells.
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Immunofluorescent staining of HeLa cells using anti-CDH2 mouse monoclonal antibody (UM500023, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
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