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XPF UltraMAB Antibody, Clone UMAB21
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human XPF (NP_005227) produced in HEK293 cell.|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||WB 1:200~500, IF 1:100, FLOW 1:100, IHC 1:500
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens excision repair cross-complementation group 4 (ERCC4)|
|ERCC11; FANCQ; RAD1; XPF|
|The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).|
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Western blot analysis of crude cell extracts (35ug) from 9 different cell lines by using anti-XPF (UMAB21) monoclonal antibody.
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-XPF monoclonal antibody (clone UMAB21, 1:500)
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-XPF (UMAB21) mouse monoclonal antibody. (UM500021, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Anti-XPF (UMAB21) mouse monoclonal antibody (UM500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF(RC223300).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB21). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB21) very specifically recognizes XPF antigen on OriGene protein microarray chip.