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ERCC4 UltraMAB Antibody, Clone UMAB20

UltraMAB™ Antibodies - Validated with 10K Protein Chip


Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
  • XPF mouse monoclonal antibody,clone UMAB20, 100 ul
$468 In Stock Add to Shopping Cart
  • XPF mouse monoclonal antibody,clone UMAB20, 30 ul
$120 In Stock Add to Shopping Cart
  • Carrier-free (BSA/glycerol-free) XPF mouse monoclonal antibody,clone UMAB20
$528 3-4 weeks Add to Shopping Cart
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Also for ERCC4 (NM_005236)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human XPF (NP_005227) produced in HEK293 cell.
Clone NameClone UMAB20 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100, IHC1:500
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ERCC excision repair 4, endonuclease catalytic subunit (ERCC4)
Alternative NameERCC11; FANCQ; RAD1; XFEPS; XPF
Database LinkNP_005227
FunctionThe protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
Related Pathway

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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-XPF monoclonal antibody (clone UMAB20, 1:500)
Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-XPF (UMAB20) mouse monoclonal antibody. (UM500020, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human uterus using anti-XPF clone UMAB20 mouse monoclonal antibody (UM500020) 1:50 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with TEE pH9.0 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear staining is seen in the epithiel cells of the uterine gland
Immunofluorescent staining of MDCK cells using anti-XPF mouse monoclonal antibody (UM500020, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB20). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB20) very specifically recognizes XPF antigen on OriGene protein microarray chip.


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