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UltraMAB

BUB1B UltraMAB Antibody, Clone UMAB7

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500007
  • anti-BUB1B mouse monoclonal antibody, clone UMAB7, 100 ul
468 In Stock Add to Shopping Cart
UM570007
  • anti-BUB1B mouse monoclonal antibody, clone UMAB7, 30 ul
$120 In Stock Add to Shopping Cart
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
WB(1)
IHC(1)
IF(2)
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Also for BUB1B (NM_001211)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
SynonymsBub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
ImmunogenProtein expressed in 293T cell transfected with human BUB1B expression vector
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB7 IsotypeIgG2a
Species ReactivityHuman, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100, IF 1:100
BackgroundThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody (Clone UMAB7) at 1:500.
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IF Image
Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
IF Image
Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

 

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