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BUB1B UltraMAB Antibody, Clone UMAB7
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for BUB1B (NM_001211)
|Protein expressed in 293T cell transfected with human BUB1B expression vector|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100, IF 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)|
|Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1|
|This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody (Clone UMAB7) at 1:500.
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.