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UltraMAB

BID UltraMAB Antibody, Clone UMAB139

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (1) Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500101
  • BID mouse monoclonal antibody,clone UMAB139, 100 ul
$468 In Stock Add to Shopping Cart
UM570101
  • BID mouse monoclonal antibody,clone UMAB139, 30 ul
$120 In Stock Add to Shopping Cart
UM500101CF
  • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone UMAB139
$528 3-4 weeks Add to Shopping Cart
CE IVD Logo CE marked antibody of this clone also available.
WB(4)
Data Img
IHC(5)
Data Img

OriGene Data

ImmunogenFull length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
Clone NameClone UMAB139 IsotypeIgG2b
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens BH3 interacting domain death agonist (BID), transcript variant 2
Alternative NameFP497
Database LinkNP_001187
FunctionThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of  HEK293 cell lysate (35ug) by using anti-BID monoclonal antibody.
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Western blot analysis of  COLO205 cell lysate (35ug) by using anti-BID monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BID monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of DU145 cell lysate (35ug) by using anti-BID monoclonal antibody UM500101
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human kidney carcinoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
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Immunohistochemical staining of paraffin-embedded human melanoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.
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Immunohistochemical staining of paraffin-embedded of 3 human colon cancers using anti-BID clone UMAB139 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500101 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The composit image of 3 colon cancers labeled A, B, and C shows strong membranous and cytoplasmic staining in >75 % of tumor cells and image A and B shows <10% nuclear stain.
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