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EGFR UltraMAB Antibody, Clone UMAB95
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for EGFR (NM_201283)
|Full length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.|
|Human, Monkey, Dog
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||WB 1:500, IHC 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3|
|ERBB; ERBB1; HER1; mENA; NISBD2; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]. |
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EGFR monoclonal antibody (Clone UMAB95).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human placenta with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.o pressure chamber. Placenta known to express high levels in the cytoplasm and membrane trophoblastic cells
Immunohistochemical staining of paraffin-embedded human lung cancer with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.0 pressure chamber. Expression in lung cancer cells are cytoplasmic and membraneous
Immunofluorescent staining of HeLa cells using anti-EGFR mouse monoclonal antibody (UM500070, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EGFR mouse monoclonal antibody (UM500070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.