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ERG UltraMAB Antibody, Clone UMAB77
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human ERG(NP_891548) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|| IHC 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens v-ets avian erythroblastosis virus E26 oncogene homolog (ERG), transcript variant 1|
|This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan |
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Western blot of cell lysates (35ug) from 8 different cell lines (1: HeLa, 2: SV-T2, 3: A549. 4: COS7, 5: Jurkat, 6: MDCK, 7: PC-12, 8: MCF7).
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ERG mouse monoclonal antibody. (UM500065; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC staining of paraffin-embedded carcinoma of human prostate using anti-ERG clone UMAB77 at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500056 requires heat-induced epitope retrieval with EDTA (pH8.7) at 95-100C 20 min. The composit image shows 3 prostate tumors with no staining in tumor cells of figure A and B although strong nuclear staining is present in the endothelial cells. Figure C shows strong nuclear with weak cytoplasmic staining in prostate tumor cells.
Immunofluorescent staining of HeLa cells using anti-ERG mouse monoclonal antibody (UM500065, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERG mouse monoclonal antibody (UM500065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.