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CD3E UltraMAB Antibody, Clone UMAB54
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for CD3E (NM_000733)
|Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD3e molecule, epsilon (CD3-TCR complex) (CD3E)|
|IMD18; T3E; TCRE|
|The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. |
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody (UM500048, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.