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UltraMAB

CD3E UltraMAB Antibody, Clone UMAB54

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500048
  • CD3E mouse monoclonal antibody,clone UMAB54, 100 ul
$468 In Stock Add to Shopping Cart
UM570048
  • CD3E mouse monoclonal antibody,clone UMAB54, 30 ul
$120 In Stock Add to Shopping Cart
WB(2)
Data Img
IHC(4)
Data Img
IF(1)
Data Img
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OriGene Data

ImmunogenFull length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
Clone NameClone UMAB54 IsotypeIgG2b
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC 1:100, IF 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CD3e molecule (CD3E)
Alternative NameIMD18; T3E; TCRE
Database LinkNP_000724
FunctionThe protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD3E monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UM500048 mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-UM500048mouse monoclonal antibody. (UM500048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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IHC staining of FFPE human tonsil using anti-CD3E clone UMAB48 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. UM500048 requires heat-induced epitope retrieval with Citrate pH6.O. The image shows strong membranous and cytoplasmic staining.
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Immunofluorescent staining of Jurkat cells using CD3E mouse monoclonal antibody (UM500048, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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