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UltraMAB

L1CAM UltraMAB Antibody, Clone UMAB47

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500043
  • L1CAM mouse monoclonal antibody, clone UMAB47, 100 ul
$468 In Stock Add to Shopping Cart
UM570043
  • L1CAM mouse monoclonal antibody, clone UMAB47, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
Data Img
IHC(7)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
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Also for L1CAM (NM_000425)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.
Clone NameClone UMAB47 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested Dilutions IHC 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens L1 cell adhesion molecule (L1CAM), transcript variant 1
Alternative NameCAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
Database LinkNP_000416
FunctionThe protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq, Jul 2008].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of cell lysates (35ug) from HeLa. Diluation: 1:500
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Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-L1CAM mouse monoclonal antibody. (UM500043; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-L1CAM mouse monoclonal antibody. (UM500043; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human ovcarian carcinoma using anti-L1CAM clone UMAB47 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500043); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen in tumor cells.
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Immunohistochemical staining of paraffin-embedded human pancreas using anti-L1CAM clone UMAB47 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (UM500043); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen glandular cells.
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Immunohistochemical staining of paraffin-embedded human melanoma using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining seen in staining in tumor cells.
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Immunohistochemical staining of paraffin-embedded human brain using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining seen in staining in granular layer and the molecular layer.
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Immunohistochemical staining of paraffin-embedded human pituitary using anti-L1CAM clone UMAB47 mouse monoclonal antibody (UM500043) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows only a subset of cells with positive cytoplasmic and membraneous stain.
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Confocal immunofluoresce image of primary rat neurons labeled with anti-L1CAM mouse monoclonal antibody (UM500043, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-L1CAM mouse monoclonal antibody (UM500043). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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