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UltraMAB

HER2 UltraMAB Antibody, Clone UMAB34

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500034
  • Purified HER2 (ERBB2) mouse monoclonal antibody,clone UMAB34, 100 ul
468 In Stock Add to Shopping Cart
UM570034
  • ERBB2 mouse monoclonal antibody,clone UMAB34, 30 ul
$120 In Stock Add to Shopping Cart
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
WB(3)
IHC(2)
IF(2)
10K-CHIP(1)
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Also for HER2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
SynonymsCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
ImmunogenHuman recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB34 IsotypeIgG2b
Species ReactivityHuman, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB:1:500, IHC:1:50, IF:1:100
BackgroundThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500034, clone UMAB34, 1:500).
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB34) at 1:500.
WB Image
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-HER2 monoclonal antibody (clone UMAB34, 1:500)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB34, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) using anti-ERBB2mouse monoclonal antibody. (Clone UMAB34, dilution 1:100; heat-induced epito
IF Image
Anti-ERBB2 mouse monoclonal antibody (UM500034, clone UMAB34, 1:100) immunofluorescent staining of COLO205 cells.
IF Image
Anti-ERBB2 mouse monoclonal antibody (UM500034, clone UMAB34, 1:100) immunofluorescent staining of MCF7 cells
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB34). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB34) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.

 

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