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UltraMAB

SQSTM1 UltraMAB Antibody, Clone UMAB13

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500013
  • SQSTM1 mouse monoclonal antibody, clone UMAB13, 100 ul
$468 In Stock Add to Shopping Cart
UM570013
  • SQSTM1 mouse monoclonal antibody, clone UMAB13, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
IHC(5)
IF(2)
10K-CHIP(1)
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Also for SQSTM1 (NM_003900)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens sequestosome 1 (SQSTM1), transcript variant 1
SynonymsA170; OSIL; p60; p62; p62B; PDB3; ZIP3
ImmunogenFull length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB13 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:200~500, IF 1:100, Flow 1:100, IF 1:100
BackgroundThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq].

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (Clone UMAB13) at 1:500.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, Dilution 1:100)
IHC Image
Immunohistochemical staining of paraffin-embedded human liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, Dilution 1:100)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of human liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, Dilution 1:100)
IHC Image
Immunohistochemical staining of paraffin-embedded human lung tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, Dilution 1:100)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of human lung tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, Dilution 1:100)
IF Image
Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody (UM500013, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
IF Image
Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody (UM500013, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
10K-CHIP Image
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SQSTM1 mouse monoclonal antibody (Clone UMAB13). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SQSTM1 (Clone UMAB13) very specifically recognizes SQSTM1 antigen on OriGene protein microarray chip.

 

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