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UltraMAB

SQSTM1 UltraMAB Antibody, Clone UMAB12

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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SKU Description Datasheet Price Availability*  
UM500012
  • SQSTM1 mouse monoclonal antibody,clone UMAB12, 100 ul
$468 In Stock Add to Shopping Cart
UM570012
  • SQSTM1 mouse monoclonal antibody,clone UMAB12, 30 ul
$120 In Stock Add to Shopping Cart

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details

WB(1)
Data Img
IHC(4)
Data Img
IF(2)
Data Img
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Also for SQSTM1 (NM_003900)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
Gene NameHomo sapiens sequestosome 1 (SQSTM1), transcript variant 1
SynonymsA170; OSIL; p60; p62; p62B; PDB3; ZIP3
ImmunogenFull length human recombinant protein of human SQSTM1(NP_003891) produced in HEK293T cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB12 IsotypeIgG2b
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100
BackgroundThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq].

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (Clone UMAB12) at 1:500.
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Immunohistochemical staining of paraffin-embedded colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded prostate tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody (UM500012, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody (UM500012, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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