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ERCC1 UltraMAB Antibody, Clone 4F9
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for ERCC1 (NM_001983)
|Protein expressed in 293T cell transfected with human ERCC1 expression vector|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2|
|COFS4; RAD10; UV20|
|The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (Clone 4F9).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded carcinoma of lung tissue (large cell) using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, dilution 1:200; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris Buffer, pH 8.0, 110C for 3min)
Immunohistochemical staining of formalin fixed paraffin-embedded carcinoma of lung tissue (squamous cell) using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, dilution 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris Buffer, pH 8.0, 110C for 3min)
Immunohistochemical staining of paraffin-embedded adenocarcinoma of lung (bronchioloalveolar) using anti-ERCC1 mouse monoclonal antibody. (Clone 4F9, dilution 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris Buffer, pH 8.0, 110C for 3min)
Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (UM500008, Clone 4F9).
Immunofluorescent staining of HeLa cells using anti-ERCC1 mouse monoclonal antibody (UM500008, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERCC1 mouse monoclonal antibody (Clone 4F9). The positive reactive proteins are highlighted with red arrows in the enlarged subarray. Other positive controls spotted in this subarray are serial dilutions of mouse IgG as controls.