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GFAP UltraMAB Antibody, Clone UMAB5
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for GFAP (NM_002055)
|Full length human recombinant protein of human GFAP (NP_002046) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB 1:500~1000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1|
|This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GFAP monoclonal antibody (Clone UMAB5).
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-GFAP monoclonal antibody (clone UMAB5, 1:500)
Immunohistochemical staining of paraffin-embedded Human brain tissue using anti-GFAP mouse monoclonal antibody (Clone UMAB5; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min).
Immunohistochemical staining of paraffin-embedded Human embryonic brain tissue using anti-GFAP mouse monoclonal antibody (Clone UMAB5; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min).
Immunofluorescent staining of PC-12 cells using GFAP mouse monoclonal antibody (UM500005, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Confocal immunofluoresce image of primary rat neurons labeled with anti-GFAP mouse monoclonal antibody (UM500005, green, 1:100) and with DAPI (blue) for nuclear. Scale bar, 10um.