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Home Antibody UltraMAB

UltraMAB

KRT19 UltraMAB Antibody, Clone UMAB2

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500002
  • anti-CK19 mouse monoclonal antibody,clone UMAB2, 100 ul
$468 In Stock Add to Shopping Cart
UM570002
  • anti-CK19 mouse monoclonal antibody,clone UMAB2, 30 ul
$120 In Stock Add to Shopping Cart
CE marked antibody of this clone also available.
WB(3)
Data Img
IHC(13)
Data Img
IF(1)
Data Img
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Also for KRT19 (NM_002276)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenRecombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
Clone NameClone UMAB2 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500~1000, IHC 1:50, IF 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens keratin 19, type I (KRT19)
Alternative NameCK19; K19; K1CS
Database LinkNP_002267
FunctionKeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CK19 monoclonal antibody (Clone UMAB2) at 1:500.
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HEK293T were transfected with 55 different plasmids of CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 antibody. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative. (1:500)
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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CK19 monoclonal antibody (clone UMAB2, 1:500)
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Immunohistochemical staining of paraffin-embedded breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human liver tissue using anti-KRT19 mouse monoclonal antibody. Anti-KRT19 clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min to produce strong staining on the bile duct and no staining in the hepatocytes.
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Immunohistochemical staining of paraffin-embedded lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-KRT19mouse monoclonal antibody. Clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min to produce strong staining on the exocrine glandular cells and no staining in the Islets of Langerhans
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of MCF7 cells using anti-CK19 mouse monoclonal antibody (UM500002, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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