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KRT8/CK8 UltraMAB Antibody, Clone UMAB1
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for KRT8/CK8 (NM_002273)
|Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||WB 1:500~1000, IHC 1:50, IF 1:50~100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens keratin 8, type II (KRT8), transcript variant 2|
|CARD2; CK-8; CK8; CYK8; K2C8; K8; KO|
|Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (Clone UMAB1) at 1:500.
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
Western blot of mouse tissue lysates (20ug) from Colon. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT8mouse monoclonal antibody. (Clone UMAB1, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of A549 cells using anti-CK8 mouse monoclonal antibody (UM500001, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT8 mouse monoclonal antibody (Clone UMAB1). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT8 (Clone UMAB1) very specifically recognizes KRT8 antigen on OriGene protein microarray chip.