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Anti-ULK3 Antibody EPR4888
Also for ULK3 (NM_001099436)
|A synthetic peptide corresponding to residues near the C-terminus of human ULK3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:25 - 1:50; FC: 1:10 - 1:100; IHC-P: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens unc-51 like kinase 3 (ULK3), transcript variant 1|
|ULK3 is a serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. It may exert this function by promoting GLI1 nuclear localization. ULK3 phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently (1).|
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Western blot - Anti-ULK3 antibody [EPR4888]; All lanes : Anti-ULK3 antibody [EPR4888] at 1/1000 dilution.Lane 1 : Human heart lysate.Lane 2 : Human testis lysate.Lane 3 : Human fetal kidney lysate.Lane 4 : LnCaP cell lysate.Lane 5 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 53 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULK3 antibody [EPR4888]; ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.