Peptide with sequence C-KPRQDTAKSPHRTK, from the internal region of the protein sequence according to NP_444277.2.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.
Clone Name
Isotype
Species Reactivity
Human
Concentration
0.5ug/ul
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1-2ug/ml
Background
Related Pathway
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HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DDK (FLAG) tag probed with TA311560 (1ug/ml) in Lane A and probed with anti-DDK Tag (1:3000) in Lane C. Mock-transfected HEK293 probed with TA311560 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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