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Home Antibody All anti-UCHL1 antibodies

Anti-UCHL1 TRUEMAB Antibody clone 2B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504289
  • UCHL1 (PGP9.5) mouse monoclonal antibody, clone 2B5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401345) , 20ug Explanation
100ul 379 In Stock
CF504289
  • Carrier-free (BSA/glycerol-free) UCHL1 mouse monoclonal antibody,clone 2B5
100ug $450 3-4 Days
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(3)
FC(1)
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Also for UCHL1 (NM_004181)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.
Clone Nameclone 2B5 IsotypeIgG1
Species ReactivityHuman , Rat , Monkey Concentration0.85 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:50, FLOW 1:100,
Predicted MW Explanation 24.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)
Alternative NameHEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
Database LinkNP_004172
Entrez Gene 7345 Human
Entrez Gene 29545 Rat
FunctionThe protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009].
Related PathwayProteaseDruggable Genome Parkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (RC201803, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCHL1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
FC Image
HEK293T cells transfected with either RC201803 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UCHL1 antibody(TA504289), and then analyzed by flow cytometry.

 

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