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Home Antibody All anti-UCHL1 antibodies

Anti-UCHL1 TRUEMAB Antibody Clone OTI2B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA504289 UCHL1 (PGP9.5) mouse monoclonal antibody, clone OTI2B5 (formerly 2B5) 100ul $379 In Stock
LC401345 UCHL1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504289 Carrier-free (BSA/glycerol-free) UCHL1 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5) 100ug $450 3-4 Days
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WB(2)
IHC(3)
FC(1)
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Also for UCHL1 (NM_004181)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.
Clone NameClone OTI2B5 IsotypeIgG1
Species ReactivityHuman , Rat , Monkey Concentration0.85 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:50, FLOW 1:100,
Predicted MW Explanation 24.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)
Alternative NameHEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
Database LinkNP_004172
Entrez Gene 7345 Human
Entrez Gene 29545 Rat
FunctionThe protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009].
Related PathwayProteaseDruggable Genome Parkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (RC201803, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCHL1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504289)
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HEK293T cells transfected with either RC201803 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UCHL1 antibody(TA504289), and then analyzed by flow cytometry.
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