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Home Antibody All anti-UCHL1 antibodies

Anti-UCHL1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324710
  • Rabbit polyclonal UCHL1 Antibody (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401345) , 20ug Explanation
400ul 325 3-7 Days
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WB(2)
IHC(2)
IF(1)
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Also for UCHL1 (NM_004181)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
Clone Name IsotypeIgG
Species ReactivityHuman, Rat (Predicted: Mouse, Bovine, Pig, Monkey) Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000, IF: 1:10~50, IHC: 1:10~50
Predicted MW Explanation 24824 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)
Alternative NameHEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
Database LinkNP_004172
Entrez Gene 7345 Human
Entrez Gene 22223 Mouse
Entrez Gene 29545 Rat
Function
Related PathwayProteaseDruggable Genome Parkinson's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
UCHL1 Antibody (C-term) (Cat.# TA324710) western blot analysis in U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).
WB Image
Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody (C-term)(Cat. #TA324710).TA324710 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
IHC Image
UCHL1 Antibody (C-term) (Cat. #TA324710)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IHC Image
UCHL1 Antibody (C-term) (Cat. #TA324710)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IF Image
Fluorescent image of U251 cell stained with UCHL1 Antibody (C-term)(Cat#TA324710/SA120806AG).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37â). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37â).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37â). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

 

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