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Home Antibody All anti-UBTF antibodies

Anti-UBTF Antibody EP2741Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303729
  • Rabbit monoclonal antibody against UBF (EP2741Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC425832) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for UBTF (NM_001076683)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human UBF was used as an immunogen.
Clone NameEP2741Y IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:500; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:30 - 1:50
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 2
Alternative NameNOR-90; UBF; UBF-1; UBF1; UBF2
Database LinkNP_001070151
FunctionUpstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - UBF1 antibody [EP2741Y]; All lanes : Anti-UBF1 antibody [EP2741Y] at 1/500 dilution.Lane 1 : (A) HeLa cell lysate.Lane 2 : (B) A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 89 kDa.Observed band size : 89 kDa.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBF1 antibody [EP2741Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303729 at 1/100-1/250 dilution.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>
FC Image
Flow Cytometry - Anti-UBF1 antibody [EP2741Y]; Overlay histogram showing A431 cells stained with TA303729 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.</title><style>.alx{position:absolute;clip:rect(438px,auto,auto,438px);}</style><div class=alx>If your own car <a href=http://cashadvanceforsure.com >approved cash advance</a> contact your credit pay period.</div>

 

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