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Anti-UBE4A Antibody EPR7331
Also for UBE4A (NM_004788)
|A synthetic peptide corresponding to residues in human UBE4A was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IF
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1|
|E4; UBOX2; UFD2|
|UBE4A is a member of the U-box ubiquitin ligase family. It is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against UBE4A may be markers for scleroderma and Crohn's disease (1).|
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Western blot - Anti-Ube4a antibody [EPR7331]; All lanes : Anti-Ube4a antibody [EPR7331] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : K562 cell lysate.Lane 3 : Caco 2 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 123 kDa.Observed band size : 125 kDa .
Other-Anti-Ube4a antibody [EPR7331](ab128861); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7331]; ab128861, at 1/100 dilution, staining Ube4a in Caco 2 cells by Immunofluorescence.