Homo sapiens ubiquitin-conjugating enzyme E2S (UBE2S)
E2-EPF; E2EPF; EPF5
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human E2EPF.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.
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(LEFT)The anti-E2EPF Pab is used in Western blot to detect E2EPF in HL-60 cell lysate. (RIGHT)Western blot analysis of E2EPF (arrow) using E2EPF Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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