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Home Antibody All anti-UBE2E3 antibodies

Anti-UBE2E3 TRUEMAB Antibody Clone OTI7E8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA800082 UBE2E3 mouse monoclonal antibody, clone OTI7E8 (formerly 7E8) 100ul $379 In Stock
LC416697 UBE2E3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800082 Carrier-free (BSA/glycerol-free) UBE2E3 mouse monoclonal antibody, clone OTI7E8 (formerly 7E8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(11)
IF(1)
FC(1)
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Also for UBE2E3 (NM_006357)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.
Clone NameClone OTI7E8 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 22.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1
Alternative NameUBCH9; UbcM2
Database LinkNP_006348
Entrez Gene 10477 Human
Entrez Gene 22193 Mouse
Entrez Gene 295686 Rat
Entrez Gene 704530 Monkey
Entrez Gene 612823 Dog
FunctionThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Related Pathway Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800082)
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Figure from citation: Immunofluorescence of UBE2E3 protein level by using anti-UBE2E3 antibody in HEK293E cells. Dilution: 1:150-500 View Citation
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HEK293T cells transfected with either RC210328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2E3 antibody(TA800082), and then analyzed by flow cytometry.
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