TrueMAB™ Monoclonal Antibodies

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To advance proteomic studies demands high quality antibodies for the detection of native epitopes of endogenous proteins. OriGene has developed TrueMAB™ monoclonal antibodies using authentic human proteins as antigens to advance the sensitivity and specificity of immunoassays. (superior performances compared to peptide-derived antibodies)

• Superior monoclonal antibodies recognize epitopes of proteins’ native conformations
• Made against authentic protein antigens (mostly full-length proteins expressed in human cell lines)
• Extensive validation
  • Western blot on cell lysates (9 cell lines with a positive control)
  • Immunohistochemistry on 24 human normal and tumor FFPE tissues
  • Immunofluorescent staining
  • Flow cytometry
  • Immuno-precipitation
• Includes a free positive control
• Multiple TrueMAB™ monoclonal antibodies available for a single protein target for cross-reference validation

TrueMAB™ monoclonal antibodies were generated using recombinant human proteins as antigens (mostly full-length proteins expressed in human cell lines) that were affinity purified under native condition to preserve the protein conformations. The commonly available commercial antibodies were generated using short peptides. The peptide antigens are inadequate to mimic the protein conformations due to the lack of three-dimensional structures. In particular, peptide-derived antibodies cannot recognize conformational epitopes which are primarily presented on the surfaces of native proteins.

In comparison to peptide-derived antibodies, TrueMAB™ monoclonal antibodies provide high sensitivity and specificity for the recognition of native epitopes appeared on the protein conformational structures. They are great tools for immunoassays that are sensitive to proteins’ conformations, such as immunofluorescence, immunoprecipitation, flow cytometry, ELISA, immunohistochemistry, high content screening (HCS), antibody arrays and more. For further questions, please send emails to antibody@origene.com or contact technical supports.

Native epitopes: The epitopes presented on the surfaces of protein’s native conformations. A. Conformational epitope (discontinuous epitope) ; B. Linear epitope. Conformational epitopes are abundant on the surfaces of native proteins. The peptide antigens are inadequate to mimic the protein conformations due to the lack of three-dimensional structures.

Epitope recognitions & applications

• Linear epitopes of denatured proteins: Western blot
• Native epitopes of proteins’ conformations (linear and conformational epitopes): Immunofluorescence, flow cytometry, immuno-precipitation, ChIP, ELISA, high content screening (HCS), antibody arrays & Luminex multiplexing
• Conformational and linear epitopes of native/partially-unfolded proteins (fixed & cross-linked): Immunohistochemistry: