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Anti-TYRO3 TRUEMAB Antibody Clone OTI2D11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for TYRO3 (NM_006293)
|Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.|
|WB, IHC, IF, IP
||WB 1:2000, IHC 1:150, IF 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens TYRO3 protein tyrosine kinase (TYRO3)|
|BYK; Dtk; Etk-2; Rek; RSE; Sky; Tif|
Entrez Gene 7301 Human
|The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. [provided by RefSeq]. |
|Protein KinaseDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500417)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500417)
Anti-TYRO3 mouse monoclonal antibody (TA500417) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.