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Anti-TUBB Antibody EPR1330
Also for TUBB (NM_178014)
|A synthetic peptide corresponding to residues in human Beta-Tubulin was used as an immunogen.|
|Mouse, Rat, Human, African Green Monkey
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens tubulin, beta class I (TUBB), transcript variant 2|
|CDCBM6; M40; OK/SW-cl.56; TUBB1; TUBB5|
Entrez Gene 203068 Human
Entrez Gene 574113 Monkey
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is posttranslationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).|
|Druggable Genome Gap junctionPathogenic Escherichia coli infection|
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Western blot - beta Tubulin antibody [EPR1330]; All lanes : Anti-beta Tubulin antibody [EPR1330] at 1/1000 dilution.Lane 1 : MCF-7 cell lysates .Lane 2 : Jurkat cell lysates .Lane 3 : Ramos cell lysates .Lane 4 : HeLa cell lysates .Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 49 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [EPR1330]; TA306985 at 1/250 dilution staining beta Tubulin in Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [EPR1330]; TA306985 at 1/250 dilution staining beta Tubulin in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-beta Tubulin antibody [EPR1330](TA306985); TA306985 showing positive staining in Normal kidney tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-beta Tubulin antibody [EPR1330](TA306985); TA306985 showing positive staining in Breast carcinoma tissue.
Immunocytochemistry/ Immunofluorescence - beta Tubulin antibody [EPR1330]; Immunofluorescent staining of HeLa cells using TA306985 at 1/100 dilution.
Flow Cytometry - Anti-beta Tubulin antibody [EPR1330]; Overlay histogram showing HeLa cells stained with TA306985 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.