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Home Antibody All anti-TUBB antibodies

Anti-TUBB Antibody EPR1330

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA306985 Rabbit monoclonal antibody against Beta-Tubulin(clone EPR1330) 100ul $325 In Stock
LC403597 TUBB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(4)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Beta-Tubulin was used as an immunogen.
Clone NameEPR1330 IsotypeIgG
Species ReactivityMouse, Rat, Human, African Green Monkey ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100 - 1:1000
Predicted MW Explanation 49 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tubulin, beta class I (TUBB), transcript variant 2
Alternative NameCDCBM6; M40; OK/SW-cl.56; TUBB1; TUBB5
Database LinkNP_821133
Entrez Gene 203068 Human
Entrez Gene 574113 Monkey
FunctionMicrotubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is posttranslationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).
Related PathwayDruggable Genome Gap junctionPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - beta Tubulin antibody [EPR1330]; All lanes : Anti-beta Tubulin antibody [EPR1330] at 1/1000 dilution.Lane 1 : MCF-7 cell lysates .Lane 2 : Jurkat cell lysates .Lane 3 : Ramos cell lysates .Lane 4 : HeLa cell lysates .Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 49 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [EPR1330]; TA306985 at 1/250 dilution staining beta Tubulin in Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Tubulin antibody [EPR1330]; TA306985 at 1/250 dilution staining beta Tubulin in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-beta Tubulin antibody [EPR1330](TA306985); TA306985 showing positive staining in Normal kidney tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-beta Tubulin antibody [EPR1330](TA306985); TA306985 showing positive staining in Breast carcinoma tissue.
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Immunocytochemistry/ Immunofluorescence - beta Tubulin antibody [EPR1330]; Immunofluorescent staining of HeLa cells using TA306985 at 1/100 dilution.
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Flow Cytometry - Anti-beta Tubulin antibody [EPR1330]; Overlay histogram showing HeLa cells stained with TA306985 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
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