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Anti-TUBA1B PHOSPHO Antibody EP1334(2)Y
Also for TUBA1B (NM_006082)
|A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha-tubulin was used as an immunogen. This antibody detects alpha-tubulin phosphorylated at tyrosine 272.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:25000 - 1:50000; ICC: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens tubulin, alpha 1b (TUBA1B)|
|Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. An essential component of microtubules, alpha-tubulin is also a multigene family in many species (1). Familial amyloidosis, British type, is an autosomal dominant disease characterized by progressive dementia, spastic paralysis and ataxia. The identity of the accumulating amyloid is not known, thus preventing the definitive classification of the disease. Findings suggest that the C-terminal fragments of both alpha- and beta-tubulin are closely associated to the amyloid deposits of familial amyloidosis, British type (2). |
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Western blot - alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]; All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] at 1/50000 dilution.Lane 1 : Jurkat cell lysates, untreated.Lane 2 : Jurkat cell lysates treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 52 kDa .