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Home Antibody All anti-TSPO antibodies

Anti-TSPO Antibody EPR5384

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310909
  • Rabbit Monoclonal antibody against TSPO
  • FREE positive control: HEK293T cell transient overexpression lysate (LC424543) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for TSPO (NM_000714)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human TSPO was used as an immunogen.
Clone NameEPR5384 IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; FC: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens translocator protein (18kDa) (TSPO), transcript variant PBR
Alternative NameBPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR
Database LinkNP_000705
FunctionTSPO (Mitochondrial benzodiazepine receptor) is present mainly in the mitochondrial compartment of peripheral tissues, and it interacts with some benzodiazepines and has different affinities than its endogenous counterpart. This protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis (1).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PBR antibody [EPR5384]; All lanes : Anti-PBR antibody [EPR5384] at 1/10000 dilution.Lane 1 : U-87 MG cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A431 cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH3T3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 19 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBR antibody [EPR5384]; TA310909 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - PBR antibody [EPR5384]; TA310909 at 1/50 dilution staining PBR in A431 cells
FC Image
Flow Cytometry - Anti-PBR antibody [EPR5384]; Overlay histogram showing HepG2 cells stained with TA310909 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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