Homo sapiens tuberous sclerosis 2 (TSC2), transcript variant 1
Synonyms:
LAM; TSC4
Immunogen
A phospho specific peptide corresponding to residues surrounding Threonine 1462 of human TSC2/Tuberin was used as an immunogen. This antibody only detects TSC2/Tuberin phosphorylated at Threonine 1462.
Buffer
Store at -20 C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Tuberous sclerosis complex-2 (TSC2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Tuberin is a target for phosphorylation by several kinases including Akt, which directly phosphorylates and inactivates TSC2 on Ser 924, Thr 1462 and Thr 1518. These phosphorylations by Akt disrupt the TSC1-TSC2 complex and disturb the subcellular localization of TSC1 and TSC2. Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 newborns, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene (4).
Related Pathway
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