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Home Antibody All anti-TSC2 antibodies

Anti-TSC2 Antibody Y320

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303843
  • Rabbit monoclonal antibody against TSC2, C-term(Y320)
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human TSC2 was used as an immunogen. Predicted to detect isoform 2-4 based on sequence homology.
Clone NameY320 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:100
Predicted MW Explanation 200 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for ICC/IF or IP.

Reference Data

Target NameHomo sapiens tuberous sclerosis 2 (TSC2), transcript variant 1
Alternative NameLAM; PPP1R160; TSC4
Database LinkNP_000539
Entrez Gene 7249 Human
Entrez Gene 22084 Mouse
Entrez Gene 24855 Rat
FunctionMutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Related PathwayDruggable Genome p53 signaling pathwaymTOR signaling pathwayInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Tuberin antibody [Y320]; Anti-Tuberin antibody [Y320] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 200 kDa.Observed band size : 220 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Tuberin antibody [Y320]; Overlay histogram showing HepG2 cells stained with TA303843 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
FC Image
Flow Cytometry - Tuberin antibody [Y320]; Overlay histogram showing HepG2 cells stained with TA303843 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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