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Anti-TRIM28 Antibody EPR5216
Also for TRIM28 (NM_005762)
|A synthetic peptide corresponding to residues in human TIF-1-beta was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens tripartite motif containing 28 (TRIM28)|
|KAP1; RNF96; TF1B; TIF1B|
|TIF-1-beta is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TIF-1-beta mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. This protein localizes to the nucleus and is thought to associate with specific chromatin regions (1).|
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Western blot - KAP1 antibody [EPR5216]; All lanes : Anti-KAP1 antibody [EPR5216] at 1/10000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : A431 cell lysates.Lane 3 : PC-3 cell lysates.Lane 4 : MCF7 cell lysates.Lysates/proteins at 10 µg per lane.Predicted band size : 89 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5216]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using 1/250 ab109287.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KAP1 antibody [EPR5216]; Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/250 ab109287.
Immunocytochemistry/ Immunofluorescence - KAP1 antibody [EPR5216]; Immunofluorescent staining of HeLa cells using 1/100 ab109287.