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Anti-TRAF6 Antibody EP591Y
Also for TRAF6 (NM_145803)
|A synthetic peptide corresponding to residues in the C-term of human TRAF6 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000 - 1:10000; FC: 1:50; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens TNF receptor-associated factor 6, E3 ubiquitin protein ligase (TRAF6), transcript variant 1|
|Tumour-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) is the only TRAF family member that participates in signal transduction of both the TNF receptor superfamily and the interleukin-1 receptor /Toll-like receptor superfamily. Acting as an adapter protein and a signal transducer, TRAF6 links members of the TNF receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. (1-2). TRAF6 stimulates NFkB through interaction with CD40, TNFR, IL-1R, p75 NTR, and TRANCE receptor. Additionally, o verexpression of TRAF6 is sufficient to activate signaling pathways leading to NF-B and AP-1 in the absence of extracellular stimuli (3-5). |
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - TRAF6 antibody [EP591Y]; Anti-TRAF6 antibody [EP591Y] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.
Immunohistochemistry (Paraffin-embedded sections) - TRAF6 antibody [EP591Y]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-TRAF6
Flow Cytometry - TRAF6 antibody [EP591Y]; ab33915 staining TRAF6 in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor?488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. P : Permeabilized .US : Unstained (Red Peak) IGG RB : IgG Rabbit (Blue Peak) .TRAF6 Ab (Green Peak)