OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-TRAF3 antibodies

Anti-TRAF3 Antibody EP1730Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300994
  • Rabbit Monoclonal Antibody against TRAF3 (Clone EP1730Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403439) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human TRAF3 was used as an immunogen.
Clone NameEP1730Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-P: 1:50; WB: 1:500; IP: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens TNF receptor-associated factor 3 (TRAF3), transcript variant 1
Alternative NameCAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1
Database LinkNP_663777
FunctionHuman TRAF-3 is a signaling molecule that interacts with the cytoplasmic tails of CD40 and other TNF-receptor family members (1). The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the IL-1R/Toll and TNF receptor family to signalling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as a docking platform for a variety of regulators of these signalling pathways and are themselves often regulated at the transcriptional and posttranslational level (2). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin (3).
Related Pathway
Apoptosis
Toll-like receptor signaling pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TRAF3 antibody [EP1730Y]; Anti-TRAF3 antibody [EP1730Y] at 1/500 dilution + Molt-4 cell lysate at 10 µg.Predicted band size : 65 kDa.Observed band size : 65 kDa.Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAF3 antibody [EP1730Y]; TA300994, at 1/50 dilution, staining TRAF3 in paraffin-embedded human tonsil tissue by immunohistochemistry.
FC Image
Flow Cytometry-Anti-TRAF3 antibody [EP1730Y](TA300994); Overlay histogram showing Jurkat cells stained with TA300994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title