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Anti-TRAF3 Antibody EP1730Y
Also for TRAF3 (NM_145725)
|A synthetic peptide corresponding to residues on the N-terminus of human TRAF3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-P: 1:50; WB: 1:500; IP: Use at an assay dependent dilution; ICC: 1:100 - 1:250; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens TNF receptor-associated factor 3 (TRAF3), transcript variant 1|
|CAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1|
|Human TRAF-3 is a signaling molecule that interacts with the cytoplasmic tails of CD40 and other TNF-receptor family members (1). The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the IL-1R/Toll and TNF receptor family to signalling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as a docking platform for a variety of regulators of these signalling pathways and are themselves often regulated at the transcriptional and posttranslational level (2). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin (3). |
Toll-like receptor signaling pathway
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Western blot - TRAF3 antibody [EP1730Y]; Anti-TRAF3 antibody [EP1730Y] at 1/500 dilution + Molt-4 cell lysate at 10 µg.Predicted band size : 65 kDa.Observed band size : 65 kDa.Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAF3 antibody [EP1730Y]; ab76147, at 1/50 dilution, staining TRAF3 in paraffin-embedded human tonsil tissue by immunohistochemistry.
Flow Cytometry-Anti-TRAF3 antibody [EP1730Y](ab76147); Overlay histogram showing Jurkat cells stained with ab76147 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.