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Anti-TPPP Antibody EPR3316
Also for TPPP (NM_007030)
|A synthetic peptide corresponding to residues in human TPPP was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:50, ICC: 1:100 - 250, IHC: 1:250 - 500, WB: 1:500 - 1000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tubulin polymerization promoting protein (TPPP)|
|p24; p25; p25alpha; TPPP/p25; TPPP1|
|Tubulin polymerization-promoting protein (TPPP) is an unfolded brain-specific protein that interacts with the tubulin/microtubule system, and is enriched in human pathological brain inclusions (1). TPPP can induce aggregation of alpha-synuclein, and can accumulate in oligodendroglial cell bodies containing fibrillized alpha-synuclein multiple system atrophy (MSA), a neurodegenerative disease. TPPP is a constituent of myelin and a high-affinity ligand for myelin basic protein (MBP), and has been shown to colocalize with MBP in myelin in normal human brains (2). |
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Western blot - Tubulin Polymerization Promoting Protein antibody [EPR3316]; All lanes : Anti-Tubulin Polymerization Promoting Protein antibody [EPR3316] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : SHSY5Y cell lysate.Lane 3 : Mouse brain lysate.Lane 4 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 24 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tubulin Polymerization Promoting Protein antibody [EPR3316]; ab92305 at 1/250 dilution staining Tubulin Polymerization Promoting Protein in paraffin-embedded Human brain tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Tubulin Polymerization Promoting Protein antibody [EPR3316]; ab92305 at 1/100 dilution staining Tubulin Polymerization Promoting Protein in SH-SY5Y cells, by immunofluorescence.
Flow Cytometry - Anti-Tubulin Polymerization Promoting Protein antibody [EPR3316]; Overlay histogram showing SH-SY5Y cells stained with ab92305 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.