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P53 UltraMAB Antibody, Clone UMAB18
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Homo sapiens tumor protein p53 (TP53), transcript variant 1|
|BCC7; LFS1; P53; TRP53|
|Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.|
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
||0.5~1.0 mg/ml (Lot Dependent)
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|WB, IHC, IF
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]. |
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody (Clone UMAB18).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Clone UMAB18, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Clone UMAB18, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of A549 cells using TP53 mouse monoclonal antibody (UM500018, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.