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Anti-TP53 Antibody EP1889Y
Also for TP53 (NM_000546)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 392 of human p53 was used as an immunogen. The antibody will detect p53 phosphorylation on Serine 392.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000 - 1:10000; IP: 1:40; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt.
|Homo sapiens tumor protein p53 (TP53), transcript variant 1|
|BCC7; LFS1; P53; TRP53|
|p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair. Various serine/threonine kinases (ATM, p38, Chk) has been limplicated to p53 phospholyation. Phospholyation at serine 392 has been linked to p53 activation. Phospholyation on serine 392 also induces p53 interaction with NF kappa beta - p65 in mouse and inhibition with Cdc2 and MDM2 in human (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. p53 is frequently mutated or inactivated in about 60% of cancers. |
Delta-Notch Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-p53 (phospho S392) antibody [EP1889Y]; All lanes : Anti-p53 (phospho S392) antibody [EP1889Y] at 1/10000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : HepG2 cell lysate treated with etoposide.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.Observed band size : 53 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP1889Y]; Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma labelling p53 (phospho S392) with TA301181 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S392) antibody [EP1889Y]; Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labelling p53 (phospho S392) with TA301181 at 1/100 dilution.