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Anti-TP53 Antibody EP155Y
Also for TP53 (NM_000546)
|A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen. The antibody will detect p53 phosphorylation on Serine 392.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC/IF: 1:250 - 1:500; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens tumor protein p53 (TP53), transcript variant 1|
|BCC7; LFS1; P53; TRP53|
|p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5). |
Delta-Notch Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - p53 (phospho S392) antibody [EP155Y]; All lanes : Anti-p53 (phospho S392) antibody [EP155Y] at 1/500 dilution.Lane 1 : MCF7 cell lysate untreated.Lane 2 : MCF7 cell lysate treated with 5 ug/ml Actinomycin for 3hrs.Lane 3 : MCF7 cell lysate treated with 5 ug/ml Actinomycin for 6hrs.Lane 4 : MCF7 cell lysate treated with 5 ug/ml Actinomycin for 18hrs.Predicted band size : 44 kDa.Observed band size : 53 kDa .
Immunohistochemistry (Paraffin-embedded sections) - p53 (phospho S392) antibody [EP155Y]; Ab33889, at a 1/100 dilution, staining p53 in paraffin embedded human prostate adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - p53 (phospho S392) antibody [EP155Y]; Ab33889, at a 1/250 dilution, staining p53 in A431 cells by Immunofluorescence.