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Home Antibody All anti-TNNT2 antibodies

Anti-TNNT2 Antibody EPR3696

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307718
  • Rabbit monoclonal antibody against TNNT2 (clone EPR3696 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400130) , 20ug Explanation
100ul 325 Next Day
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WB(1)
IHC(1)
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Also for TNNT2 (NM_000364)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TNNT2 was used as an immunogen.
Clone NameEPR3696 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:20000 - 1:100000; IHC-P: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1
Alternative NameCMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC
Database LinkNP_000355
Entrez Gene 7139 Human
Entrez Gene 21956 Mouse
Entrez Gene 24837 Rat
FunctionTNNT2 is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
Related PathwayDruggable Genome Cardiac muscle contractionHypertrophic cardiomyopathy (HCM)Dilated cardiomyopathy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cardiac Troponin T antibody [EPR3696]; All lanes : Anti-Cardiac Troponin T antibody [EPR3696] at 1/20000 dilution.Lane 1 : Human heart lysate.Lane 2 : Human skeletal muscle lysate.Lane 3 : Mouse heart lysate.Lane 4 : Rat heart lysate.Lysates/proteins at 10 ug per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 36 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3696]; IHC image ofCardiac Troponin T staining inHuman Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA307718, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

 

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