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Anti-TNNI3 Antibody EP1106Y
Also for TNNI3 (NM_000363)
|A synthetic peptide corresponding to residues near the N-terminus of human Troponin 1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC: Use at an assay dependent concentration; IHC-Fr: Use at an assay dependent concentration; FC: 1:500 - 1:1000; WB: 1:100000 - 1:1e+006; IP: 1:50; ICC/IF: Use at an assay dependent concentration; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens troponin I type 3 (cardiac) (TNNI3)|
|CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1|
|Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]. |
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Western blot - cardiac Troponin I antibody [EP1106Y]; Anti-Cardiac Troponin I antibody [EP1106Y] at 1/1000000 dilution + fetal heart cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 28 kDa.
Immunohistochemistry (Paraffin-embedded sections) - cardiac Troponin I antibody [EP1106Y]; ab52862 at 1/250 dilution staining cardiac Troponin I in human cardiac muscle by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody [EP1106Y]; ab52862 stainingCardiac Troponin IinHumanH9 differentiated stem cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withparaformaldehyde, permeabilized with0.5% saponin and blocked with 5%serum for 1hour at 20°C. Samples were incubated with primary antibody (1/100 in1% saponin and 5% FBS PBS buffer) for 1 hour at 4°C. AFITC-conjugatedGoat anti-rabbit IgG polyclonal (1/400)was used as the secondary antibody.
Flow Cytometry - Anti-Cardiac Troponin I antibody [EP1106Y]; ab52862 stainingCardiac Troponin Iin Human H9 differentiated stem cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 5% FBS and 1% Saponin) for 1 hour at 4°C. A FITC-conjugatedanti-Rabbit IgG (1/300) was used as the secondary antibody.
Flow Cytometry - Anti-Cardiac Troponin I antibody [EP1106Y]; ab52862 stainingCardiac Troponin IinHuman iPSC derived Cardiomyocytesby Flow Cytometry. The sample was incubated with the primary antibody (1/600 in0.1%saponin buffer) for1 hourat 4°C. An Alexa Fluor? 488-conjugatedGoat anti-rabbit IgG (1/500) was used as the secondary antibody.Gating strategy - reference isotype.