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Home Antibody All anti-TNNI3 antibodies

Anti-TNNI3 Antibody EP1106Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303719
  • Rabbit monoclonal antibody against Troponin 1(EP1106Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424766) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(2)
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Also for TNNI3 (NM_000363)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Troponin 1 was used as an immunogen.
Clone NameEP1106Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC: Use at an assay dependent concentration; IHC-Fr: Use at an assay dependent concentration; FC: 1:500 - 1:1000; WB: 1:100000 - 1:1e+006; IP: 1:50; ICC/IF: Use at an assay dependent concentration; IHC-P: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens troponin I type 3 (cardiac) (TNNI3)
Alternative NameCMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1
Database LinkNP_000354
FunctionTroponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - cardiac Troponin I antibody [EP1106Y]; Anti-Cardiac Troponin I antibody [EP1106Y] at 1/1000000 dilution + fetal heart cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 28 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - cardiac Troponin I antibody [EP1106Y]; TA303719 at 1/250 dilution staining cardiac Troponin I in human cardiac muscle by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody [EP1106Y]; TA303719 stainingCardiac Troponin IinHumanH9 differentiated stem cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withparaformaldehyde, permeabilized with0.5% saponin and blocked with 5%serum for 1hour at 20°C. Samples were incubated with primary antibody (1/100 in1% saponin and 5% FBS PBS buffer) for 1 hour at 4°C. AFITC-conjugatedGoat anti-rabbit IgG polyclonal (1/400)was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-Cardiac Troponin I antibody [EP1106Y]; TA303719 stainingCardiac Troponin Iin Human H9 differentiated stem cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 5% FBS and 1% Saponin) for 1 hour at 4°C. A FITC-conjugatedanti-Rabbit IgG (1/300) was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-Cardiac Troponin I antibody [EP1106Y]; TA303719 stainingCardiac Troponin IinHuman iPSC derived Cardiomyocytesby Flow Cytometry. The sample was incubated with the primary antibody (1/600 in0.1%saponin buffer) for1 hourat 4°C. An Alexa Fluor? 488-conjugatedGoat anti-rabbit IgG (1/500) was used as the secondary antibody.Gating strategy - reference isotype.

 

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